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	Provedor de dados Biological Sciences (2) Autor Benevides, Norma Maria Barros (2) Queiroz, Ismael Nilo Lino de (2) Rodrigues, José Ariévilo Gurgel (2) Carneiro, José Gerardo (1) Holanda, Márjory Lima (1) Pereira, Maria Gonçalves (1) Quinderé, Ana Luíza Gomes (1) Silva Neto, Antônio Alves da (1) Souza, Ricardo Basto (1) Mais... Palavra-chave Polysulfated (2) APTT test (1) Antithrombotic agent (1) Bioactivities (1) Gelidiella acerosa (1) In vivo toxicity (1) Precipitation methods (1) Rhodophyceae (1) Rhodophyta (1) Structural analysis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Analysis of two precipitation methods on the yield, structural features and activity of sulfated polysaccharides from Gracilaria cornea (Rhodophyta) Biological Sciences Souza, Ricardo Basto; Queiroz, Ismael Nilo Lino de; Silva Neto, Antônio Alves da; Carneiro, José Gerardo; Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros. The global demand for natural products from seaweeds has increased worldwide; however, no description of the use of isoamly alcohol (IAA) for obtaining of sulfated polysaccharides (SPs) has been reported. We investigated the efficiency of two precipitation methods (M) in obtaining SPs from the red seaweed Gracilaria cornea. SPs enzymatically isolated were concentrated with cetylpyridinium chloride (M I) or IAA (M II) and extracts were examined with regard to their yield, structural features and in vitro effects on the activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193 IU mg-1). Yield difference reached 12.99%. Quantitative determination of sulfate was similar between the two methods (̴ 26%), but extracts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhodophyceae; Polysulfated; Precipitation methods; Structural analysis; APTT test. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24016 Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa Biological Sciences Queiroz, Ismael Nilo Lino de; Rodrigues, José Ariévilo Gurgel; Quinderé, Ana Luíza Gomes; Holanda, Márjory Lima; Pereira, Maria Gonçalves; Benevides, Norma Maria Barros. Metabolites isolated from Gelidiella species (Rhodophyta) have been few studied. We evaluated a sulfated polysaccharidic fraction from G. acerosa collected from two Brazilian beaches on the northwestern coast of Brazil (Flecheiras-F and Pedra Rachada-PR) on coagulation proteases and thrombosis. Their toxicity in vivo was also assessed. Enzymatic extractions yielded 1.40%, and similar chromatographic profiles (DEAE-cellulose) were obtained, with fractions (Ga-I→V) containing differences among the relative proportions of sulfate (5-42%), and revealing charge density patterns by electrophoresis. Ga-IV-PR had a discrete effect (3.01 IU mg-1) on normal human coagulation compared with heparin (193 IU mg-1) and was tested on coagulation proteases (thrombin and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhodophyta; Polysulfated; Gelidiella acerosa; Antithrombotic agent; Bioactivities; In vivo toxicity. Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23704 Registros recuperados: 2 Primeira ... 1 ... Última

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